تفاصيل الوثيقة
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نوع الوثيقة |
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مقال في مجلة دورية |
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عنوان الوثيقة |
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Postmortem genetic testing should be recommended in sudden cardiac death cases due to thoracic aortic dissection Postmortem genetic testing should be recommended in sudden cardiac death cases due to thoracic aortic dissection |
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لغة الوثيقة |
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الانجليزية |
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المستخلص |
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The main life-threatening complications of the corresponding aneurysms, are an important cause of sudden cardiac death. Despite the usefulness of the molecular diagnosis of these conditions in the clinical setting, the corresponding forensic field remains largely unexplored. The main goal of this study was to explore and validate a new massive parallel sequencing candidate gene assay as a diagnostic tool for acute thoracic aortic dissection autopsy cases.
Materials and methods Massive parallel sequencing of 22 thoracic aortic disease candidate genes performed in 17 cases of thoracic aortic dissection using AmpliSeq and Ion Proton technologies. Genetic variants were filtered by location, type, and frequency at the Exome Aggregation Consortium and an internal database and further classified based on the American College of Medical Genetics and Genomics (ACMG) recommendations published in 2015. All prioritized results were confirmed by traditional sequencing. Results From the total of 10 potentially pathogenic genetic variants identified in 7 out of the 17 initial samples, 2 of them were further classified as pathogenic, 2 as likely pathogenic, 1 as possibly benign, and the remaining 5 as variants of uncertain significance, reaching a molecular autopsy yield of 23%, approximately. Conclusions This massive parallel sequencing candidate gene
approach proved useful for the molecular autopsy of aortic dissection sudden cardiac death cases and should therefore be progressively incorporated into the forensic field, being especially beneficial for the anticipated diagnosis and risk stratification of any other family member at risk of developing the same condition. |
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ردمد |
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0937-9827 |
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اسم الدورية |
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International Journal of Legal Medicine |
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المجلد |
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1 |
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العدد |
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1 |
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سنة النشر |
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1438 هـ
2017 م |
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نوع المقالة |
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مقالة علمية |
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تاريخ الاضافة على الموقع |
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Thursday, June 1, 2017 |
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الباحثون
| Gago-Díaz Marina | Marina, Gago-Díaz | باحث | دكتوراه | |
| Ramos-Luis Eva | Eva, Ramos-Luis | باحث | دكتوراه | |
| Zoppis Silvia | Silvia, Zoppis | باحث | دكتوراه | |
| Zorio Esther | Esther, Zorio | باحث | دكتوراه | |
| Molina Pilar | Pilar, Molina | باحث | دكتوراه | |
| Braza-Boïls Aitana | Aitana, Braza-Boïls | باحث | دكتوراه | |
| Giner Juan | Juan, Giner | باحث | دكتوراه | |
| Sobrino Beatriz | Beatriz, Sobrino | باحث | دكتوراه | |
| Amigo Jorge | Jorge, Amigo | باحث | دكتوراه | |
| Blanco-Verea, Alejandro | Alejandro, Blanco-Verea, | باحث | دكتوراه | |
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الرجوع إلى صفحة الأبحاث
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